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Multi-compartment and multi-host vector suite for recombinant protein expression and purification
(Frontiers Media, 2018)
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous ...
Identification of a non-classical three-dimensional nuclear localization signal in the intestinal fatty acid binding protein
(Public Library of Science, 2020)
The intestinal fatty acid binding protein (FABP) is a small protein expressed along the small intestine that bind long-chain fatty acids and other hydrophobic ligands. Several lines of evidence suggest that, once in the ...
20S proteasome-dependent generation of an IEpp89 murine cytomegalovirus-derived H-2Ld epitope from a recombinant protein
(2007-04-06)
The majority of MHC class I epitopes is generated through the ubiquitin-proteasome system. In the present study, we have analyzed the proteasome-dependent generation of the IE pp89 MCMV-derived H-2Ld epitope by both in ...
Analysis of RecA-independent recombination events between short direct repeats related to a genomic island and to a plasmid in Escherichia coli K12
(PeerJ Inc, 2017)
RecA-independent recombination events between short direct repeats, leading to
deletion of the intervening sequences, were found to occur in two genetic models
in the Escherichia coli K12 background. The first model was ...
Functional hemichannels formed by human connexin 26 expressed in bacteria
(© 2015 The Author(s) CC-BY Licence, 2015)
Gap-junction channels (GJCs) communicate the cytoplasm of adjacent cells and are formed by head-to-head association of two hemichannels (HCs), one from each of the neighbouring cells. GJCs mediate electrical and chemical ...
Functional hemichannels formed by human connexin 26 expressed in bacteria
(© 2015 The Author(s) CC-BY Licence, 2015)
Gap-junction channels (GJCs) communicate the cytoplasm of adjacent cells and are formed by head-to-head association of two hemichannels (HCs), one from each of the neighbouring cells. GJCs mediate electrical and chemical ...
Recombinant and epitope-based vaccines on the road to the market and implications for vaccine design and production
(2016-03-03)
Novel vaccination approaches based on rational design of B- and T-cell epitopes - epitope-based vaccines - are making progress in the clinical trial pipeline. The epitope-focused recombinant protein-based malaria vaccine ...
Nasal immunization with the c-terminal domain of bcla3 induced specific igg production and attenuated disease symptoms in mice infected with clostridioides difficile spores
(MDPI AG, 2020-09)
Clostridioides difficile is a Gram-positive, spore-forming bacterium that causes a severe intestinal infection. Spores of this pathogen enter in the human body through the oral route, interact with intestinal epithelial ...